Journal: Plants

Article Title: Resveratrol Inhibits Venezuelan Equine Encephalitis Virus Infection by Interfering with the AKT/GSK Pathway

doi: 10.3390/plants10020346

Figure Lengend Snippet: 50% cytotoxic dose and effective dose (CC50 and EC50) of resveratrol against Venezuelan equine encephalitis virus (VEEV) in two distinct cell types. Vero ( A , B ) and U87MG ( C , D ) cells were treated with resveratrol at indicated concentrations (CC50) along with VEEV-TC-83 infection for 16 h at an MOI of 0.1 followed by measurement (EC50) of ( A , D ) cell viability and ( B , E ) replication of reporter VEEV-TC83luc quantified by cellular ATP and luciferase activity, respectively. The figures were plotted following the normalizing toward viral control groups. Viral control refers to no resveratrol-treated but VEEV-infected cells, while mock control was defined as cells treated with a solvent and without infection. ( E ) The CC50, EC50, and therapeutic index (TI) were estimated via non-linear regression with GraphPad Prism v7.0 to determine the 50% cell viability and effective dose. Data were presented as means ± SEM.

Article Snippet: The 50% cytotoxic concentration (CC50) values were calculated via non-linear regression fitted to the data by GraphPad Prism v7.0.

Techniques: Infection, Luciferase, Activity Assay